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本文报道了含有杂交瘤生长因子的人纤维母细胞系CRL1506和EB病毒转化经克隆化的人B淋巴母细胞系N23两种条件培养液,对分泌肾综合征出血热病毒人单克隆抗体的人-鼠杂交瘤和人—(人—鼠)杂交瘤细胞系的生长、克隆化及抗体分泌有明显促进作用,并排除了白细胞介素2和白细胞介素4作用的可能性。结合文献报道,初步认为来自这两种条件培养液的杂交瘤生长因子很可能含有白细胞介素6。正确选择促进人—鼠杂交瘤生长、克隆化和抗体分泌的条件培养液,对于克服生产人单克隆抗体杂交瘤技术所存在的融合率低、克隆化难、抗体产量少等困难具有较大的应用价值。
This paper reports the hybridoma growth factor-containing human fibroblast cell line CRL1506 and Epstein-Barr virus transformed cloned human B lymphoblastoid cell line N23 two conditioned medium, the secretion of human renal syndrome virus hemagglutinin human monoclonal antibody - mouse hybridoma and human - (human - mouse) hybridoma cell line growth, cloning and antibody secretion was significantly promoted, and ruled out the possibility of interleukin 2 and interleukin 4 role. Combined with the literature, it is preliminarily believed that the hybridoma growth factors from these two conditioned mediums probably contain interleukin-6. The correct choice of conditions to promote human-mouse hybridoma growth, cloning and antibody secretion of culture medium for the production of human monoclonal antibody hybridoma technology there is a low fusion rate, cloning, antibody yield and other difficulties have a greater Value.