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目的研究已酮可可碱(PTX)对重症急性胰腺炎(SAP)大鼠肺损伤的保护作用。方法健康成年雄性SD大鼠72只,随机分为对照(C)组、实验(E)组和干预(P)组。E组和P组用5%牛磺胆酸钠逆行胰胆管注射制备SAP模型。然后,P组立即腹腔注射PTX。于制模后2、6和12 h观察胰腺和肺组织的病理学改变;ELISA法测定血清中性粒细胞趋化因子(CINC)和白细胞介素6(IL-6)水平变化;Western blot法检测肺组织核因子抑制蛋白(IκBα)的表达量。结果E组血清CINC、IL-6水平及肺组织IκBα蛋白的表达均显著高于同时段C组(P<0.05);与E组比较,P组血清CINC、IL-6水平和肺组织IκBα蛋白表达量均明显降低(P<0.05),胰腺和肺组织病理改变减轻。结论SAP肺损伤时,肺组织IκBα明显升高,核因子κB(NF-κB)活化,从而启动CINC、IL-6等过量表达。PTX可以抑制NF-κB/IκB信号通路的激活,对SAP肺损伤具有保护作用。
Objective To investigate the protective effect of pentoxifylline (PTX) on lung injury in rats with severe acute pancreatitis (SAP). Methods Seventy-two healthy male Sprague Dawley rats were randomly divided into control group (C), experimental group (E) and intervention group (P). E and P groups with 5% sodium taurocholate pancreaticobiliary injection SAP model. Then, group P was injected intraperitoneally with PTX. The pathological changes of pancreas and lung tissues were observed at 2, 6 and 12 h after model establishment. Serum levels of neutrophil chemotactic factor (CINC) and interleukin 6 (IL-6) were measured by ELISA. Western blot The expression of nuclear factor kinin (IκBα) in lung tissue was detected. Results The serum levels of CINC and IL-6 in serum and the expression of IκBα protein in lung tissue of group E were significantly higher than those in group C at the same time (P <0.05). Compared with group E, the levels of serum CINC and IL-6 in serum and levels of IκBα The expression levels were significantly decreased (P <0.05), and the pathological changes in the pancreas and lung tissues were alleviated. Conclusions In lung injury, IκBα is significantly increased and NF-κB is activated in lung tissue, which leads to the over-expression of CINC and IL-6. PTX can inhibit the activation of NF-κB / IκB signaling pathway, which has a protective effect on SAP lung injury.