论文部分内容阅读
目的:合成Livin靶向的反义核苷酸(ASODN),观察其对前列腺癌PC3细胞凋亡和增殖等生物学特性的影响。方法:合成全硫代磷酸化修饰的Livin ASODN并通过脂质体转染前列腺癌PC3细胞。采用MTT法检测其对细胞增殖的影响,采用RT-PCR检测转染后Livin基因mRNA的表达情况,采用流式细胞仪检测转染后细胞的凋亡效应,采用体内成瘤实验比较细胞在裸鼠体内成瘤性的变化,采用激酶法测定Caspase-3活性的变化。结果:Livin ASODN转染PC3细胞后,与对照组相比,实验组细胞内Livin mRNA的表达水平下调(P<0.01);细胞的增殖受到显著抑制(P<0.01);细胞凋亡率明显增高(P<0.01);肿瘤细胞在荷瘤鼠体内的成瘤体积小于对照组(P<0.05);Caspase-3活性明显增加(P<0.05)。结论:靶向Livin基因的ASODN干扰了前列腺癌PC3细胞中Livin基因的表达,抑制了细胞的增殖并诱导其凋亡,延缓了肿瘤细胞的生长。
OBJECTIVE: To synthesize Livin-targeted antisense oligonucleotide (ASODN) and observe its effects on the biological characteristics of prostate cancer PC3 cells such as apoptosis and proliferation. Methods: The peritrophosphorylation of Livin ASODN was synthesized and transfected into prostate cancer PC3 cells by liposome. The effect of Livin gene mRNA expression was detected by RT-PCR. The apoptosis effect of transfected cells was detected by flow cytometry. The cells in nude The changes of tumorigenicity in mice were measured by the kinase method to determine the changes of Caspase-3 activity. Results: Compared with the control group, the expression of Livin mRNA in the experimental group was significantly decreased (P <0.01), the proliferation of the cells was significantly inhibited (P <0.01), and the apoptosis rate of Livin ASODN transfected PC3 cells was significantly increased (P <0.01). The tumor volume of tumor cells in tumor-bearing mice was smaller than that in control group (P <0.05), and the activity of Caspase-3 was significantly increased (P <0.05). CONCLUSION: ASODN targeting Livin gene interferes with the expression of Livin gene in prostate cancer PC3 cells, inhibits cell proliferation and induces apoptosis, and delays the growth of tumor cells.