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目的:探讨新型全反式维甲酸衍生物4氨基-2三氟甲基苯基维甲酸酯(4-amino-2-triluoromethyl-phenyl retinate,ATPR)对人脑胶质瘤U87细胞增殖及分化的影响。方法:不同浓度的ATPR作用U87细胞后,分别采用细胞计数试剂盒-8(cell counting kit-8,CCK-8)法及平板克隆形成实验检测其对细胞增殖的影响;光学显微镜和电子显微镜下观察细胞形态的改变;FCM法检测对细胞周期分布的影响;蛋白质印迹法检测分化指标胶质纤维酸性蛋白(glial ibrillary acidic protein,GFAP)的表达。结果:ATPR对U87细胞的增殖抑制作用呈浓度相关性,单细胞克隆形成率下降,细胞形态趋于成熟;FCM法检测结果显示,G0/G1期细胞所占比例增加、S期细胞所占比例减少,细胞呈G0/G1期阻滞;蛋白质印迹法检测结果显示,GFAP蛋白表达量上升。结论:ATPR对人脑胶质瘤U87细胞有一定增殖抑制作用并诱导其分化。
AIM: To investigate the effects of 4-amino-2-triluoromethyl-phenyl retinate (ATPR), a novel all-trans retinoic acid derivative, on the proliferation and differentiation of human glioma U87 cells Impact. Methods: U87 cells were treated with different concentrations of ATPR. Cell proliferation kit was detected by cell counting kit-8 (CCK-8) assay and plate clone formation assay respectively. Under light microscope and electron microscope The morphological changes of cells were observed. The effect of FCM on the cell cycle distribution was examined. The expression of glial fibrillary acidic protein (GFAP) was detected by Western blotting. Results: The inhibitory effect of ATPR on the proliferation of U87 cells was concentration-dependent, the rate of single cell colony formation decreased and the cell morphology tended to mature. The FCM assay showed that the proportion of cells in G0 / G1 phase increased, the proportion of S phase cells Decreased, G0 / G1 cell arrest; Western blot results showed that GFAP protein expression increased. Conclusion: ATPR can inhibit the proliferation of human glioma U87 cells and induce their differentiation.