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以细胞存活率、逸出酶(GOT,LDH)和酶组织化学为指标,观察四氯化碳等14种化合物对大鼠游离肝细胞的毒性作用。发现,细胞存活率随6种卤代烃染毒浓度加大而降低,其相对毒性大小也与体内毒性大小顺序大致相符。四氯化碳等3种卤代烃体外染毒使肝细胞悬液上清液中GOT,LDH活性升高;铬酸钠等化合物却导致酶活性下降。14种化合物都对一种或一种以上肝细胞酶活性表现抑制作用。
Cell viability, escaping enzyme (GOT, LDH) and enzyme histochemistry were used as indexes to observe the toxic effects of 14 compounds such as carbon tetrachloride on isolated hepatocytes in rats. It was found that the cell viability decreased with the concentration of 6 kinds of halohydrocarbons increased, and its relative toxicity also roughly in accordance with the order of the body toxicity. Carbon tetrachloride and other three kinds of halogenated hydrocarbons in vitro exposure to liver cell suspension supernatant GOT, LDH activity increased; sodium chromate and other compounds led to decreased enzyme activity. All 14 compounds showed inhibitory activity on one or more of the hepatocytes’ enzymatic activity.