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The mechanism of apoptosis induced by nicotinamide was investigated by treating mouse mye- loma cells (Sp2/0) with various concentrations of nicotinamide. The typical hallmarks of apoptosis, including chromatin condensation and DNA fragmentation, were detected when cells were treated with nicotinamide at concentrations of 30, 40, 50, and 60 mmol/L. The apoptosis percentage increased with increasing nicoti- namide concentration. Interestingly, the strong antioxidant melatonin did not restrain the apoptosis induced by nicotinamide in mouse myeloma cells but greatly increased the induction of nicotinamide on apoptosis. When cells were preincubated with 0.1, 1, and 10 mmol/L melatonin before nicotinamide induction, the per- centage of apoptosis induced by 50 mmol/L nicotinamide markedly increased with increasing melatonin concentration. These results suggest that apoptosis induced by nicotinamide has no relationship with oxida- tive stress and melatonin could enhance nicotinamide-induced apoptosis in mouse myeloma cells by stimu- lating cell division in a certain manner. Nicotinamide may provide a new method to treat some kinds of tu- mors with no damage to normal tissues.
The mechanism of apoptosis induced by nicotinamide was investigated by treating mouse mye-loma cells (Sp2 / 0) with various concentrations of nicotinamide. The typical hallmarks of apoptosis, including chromatin condensation and DNA fragmentation, were detected when cells were treated with nicotinamide at concentrations of 30, 40, 50, and 60 mmol / L. Interestingly, the strong antioxidant melatonin did not restrain the apoptosis induced by nicotinamide in mouse myeloma cells but greatly increased the induction of nicotinamide on These cells were preincubated with 0.1, 1, and 10 mmol / L melatonin before nicotinamide induction, the per- centage of apoptosis induced by 50 mmol / L nicotinamide markedly increased with increasing melatonin concentration. These results suggest that apoptosis induced by nicotinamide has no relationship with oxida- tive stress and melatonin could enhance nicotinamide-induced a poptosis in mouse myeloma cells by stimu- lating cell division in a certain manner. Nicotinamide may provide a new method to treat some kinds of tu- mors with no damage to normal tissues.