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应用免疫组织化学方法,研究了P物质免疫反应纤维在实验性哮喘豚鼠下呼吸道的变化。实验组一抗最佳稀释浓度为1:5000,对照组为1:3000。当一抗浓度均为1:5000时,两组的P物质反应纤维见于气管和支气管平滑肌层、上皮和外膜层,肺内各级支气管平滑肌层、肺内各级血管壁内及其周围结缔组织和肺泡隔内,但实验组下呼吸道各部组织的P物质阳性纤维数量明显多于对照组。这些结果提示:P物质可能参与哮喘发作的病理生理机制,是其发作的因素之一。
Immunohistochemistry was used to study the changes of substance P immunoreactive fibers in the lower respiratory tract of experimental asthmatic guinea pigs. The optimal dilution of the primary antibody in the experimental group was 1:5000 and the control group was 1:3000. When the primary antibody concentration was 1:5000, the substance P reactive fibers in the two groups were found in the tracheal and bronchial smooth muscle layers, epithelial and adventitial layers, in the lungs at various levels of the bronchial smooth muscle layer, in and around the pulmonary blood vessel walls at all levels. In the tissues and alveolar septae, the amount of substance P-positive fibers in the lower respiratory tract tissue of the experimental group was significantly greater than that of the control group. These results suggest that substance P may be involved in the pathophysiological mechanism of asthma attacks and is one of the factors responsible for its onset.