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目的应用组织芯片技术探讨人类肺癌中EphB4、HIF-1α和IGF-Ⅱ的表达情况以及三者表达的生物学意义。方法构建一个包含54例肺癌和10例正常肺组织的组织芯片蜡块,应用免疫组化SP法检测其EphB4、HIF-1α和IGF-Ⅱ蛋白的表达情况。结果病例组中EphB4、HIF-1α和IGF-Ⅱ蛋白的阳性率分别是44.4%、46.3%和42.6%,显著高于正常对照组(P<0.05);这三种蛋白的表达与肿瘤的大体类型、分化程度和TNM分期密切相关(P<0.05),而与患者性别、年龄、有无淋巴结转移无关(P>0.05)。三种蛋白的表达彼此之间存在显著正相关关系(P<0.01)。结论EphB4、HIF-1α和IGF-Ⅱ蛋白的表达可能与肿瘤的发生和恶性行为有关,三者之间存在着相互作用。
Objective To investigate the expression of EphB4, HIF-1α and IGF-Ⅱ in human lung cancer and the biological significance of their expression using tissue microarray. Methods A tissue chip containing 54 lung carcinomas and 10 normal lung tissues was constructed. The expression of EphB4, HIF-1α and IGF-Ⅱ protein was detected by immunohistochemical SP method. Results The positive rates of EphB4, HIF-1α and IGF-Ⅱ in cases were 44.4%, 46.3% and 42.6%, respectively, which were significantly higher than those in normal controls (P <0.05). The expressions of these three proteins were significantly correlated with tumor size Type, degree of differentiation and TNM stage (P <0.05), but not with gender, age, lymph node metastasis (P> 0.05). There was a significant positive correlation between the three proteins expression (P <0.01). Conclusion The expression of EphB4, HIF-1α and IGF-Ⅱ may be related to tumorigenesis and malignant behavior, and there is an interaction between them.