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目的探讨精浆免疫抑制物质（ＳＰＩＭ）对小鼠免疫功能的影响方法从正常男性精液中提取精浆免疫抑制物质．从ＢＡＬＢ／Ｃ雄性小鼠收集腹腔巨噬细胞（Ｍ）．通过Ｍ－ＥＡ花环试验和Ｍ—ＹＣ花环试验观察ＳＰＩＭ不同剂量及不同作用时间对小鼠腹腔Ｍ表面的Ｆｃ受体（ＦｃＲ）和Ｃ３ｂ受体（Ｃ３ｂＲ的影响．结果ＳＰＩＭ作用２４ｈ，小鼠腹腔ＭＥＡ和ＹＣ花环形成率分别为４２±４．６３和４０±５．３９，而７２ｈ分别为７４±７．９６和５９±３．４３．ＳＰＩＭＩ１５０μｇ组的ＥＡ和ＹＣ花环形成率分别为４２±１．７２和３９±２．６５，对照组则为７０±２．１３和７２±２．８７．结论ＳＰＩＭ对小鼠腹腔Ｍ的ＦｃＲ和Ｃ３ｂＲ的抑制作用有剂量依赖关系，并且以作用２４ｈ后其抑制作用达到高峰。
Objective To investigate the effect of immunosuppressant (SPIM) on immune function in mice.Methods Seminal plasma immunosuppressive substances were extracted from normal male semen. Peritoneal macrophages (M) were harvested from BALB / C male mice. The effect of SPIM at different doses and different time on the Fc receptor (FcR) and C3b receptor (C3bR) in the peritoneal M surface was observed by M-EA rosette test and M-YC rosette test.Results The effect of SPIM on the abdominal cavity The formation rates of MEA and YC rosette were 42 ± 4.63 and 40 ± 5.39, respectively, while those in 72 h were 74 ± 7.96 and 59 ± 3.43, respectively.The rates of formation of EA and YC rosette in SPIMI 150 μg group were 42 ± 1 .72 and 39 ± 2.65 in the control group and 70 ± 2.13 and 72 ± 2.87 in the control group.Conclusion SPIM can inhibit the FcR and C3bR of M in mice in a dose-dependent manner, and after 24h Inhibition peaked.