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由于目前孕酮被广泛用于高危妊娠处理,作者试图了解孕酮处理后对新生儿的影响,本文中作者对10名新生儿进行了遗传学分析,这些新生儿的母亲在妊娠期间都接受过连续的孕酮治疗达3个月或直至分娩,剂量为肌注孕酮25mg+烯丙雌烯醇2×5mg/天(organon;17α-allylestr-4-en-17-β-ol)。对照组10名新生儿是正常妊娠分娩的,其母亲妊娠期间未接受过孕酮治疗。所有新生儿生后24小时内用改进的Edwards方法进行分析,用FPG技术进行harlequin差别染色,细胞培养中的BUdR浓度为5μg/ml。在三个不同的固定时间(48、72和96h)对培养物进行观察以确定细胞动力学是否有差异。每一组中共分析1,000个第二次分裂
Since progesterone is currently widely used in high-risk pregnancies, the authors attempted to understand the effect of progesterone treatment on newborns. In this paper, the authors genetically analyzed 10 newborns whose mothers had received either during pregnancy Continuous treatment of progesterone for up to 3 months or until delivery, the dose of intramuscular injection of progesterone 25mg + allyl estradiol 2 × 5mg / day (organ; 17α-allylestr-4-en-17-β-ol). Control group of 10 newborns is normal pregnancy delivery, the mother did not receive progesterone treatment during pregnancy. All newborns were analyzed within 24 hours of birth by the modified Edwards method and harlequin differential staining was performed using the FPG technique with a BUdR concentration of 5 μg / ml in cell culture. Cultures were observed at three different fixed times (48, 72 and 96 h) to determine if there was any difference in cell kinetics. A total of 1,000 second divisions were analyzed in each group