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背景:已有实验表明,骨髓基质细胞具有分化为骨骼、神经干细胞及造血干细胞的巨大潜能,而黄芩甙具有诱导细胞分化的作用。目的:探讨黄芩甙体外诱导骨髓基质细胞分化为神经干细胞的可能性。方法:分离培养Wistar纯系大鼠骨髓基质细胞,并将分离的骨髓基质细胞分为实验组和对照组,对照组不干预,实验组用黄芩甙(350~400μmol/L)诱导,2组的培养环境相同。持续诱导6d后选取生长良好的细胞进行蛋白质印迹和反转录PCR(RT-PCR)法检测。结果与结论:黄芩甙诱导7d后,骨髓基质细胞形成较典型的神经细胞形态。诱导前骨髓基质细胞不表达神经细胞标记蛋白mRNA和蛋白;诱导6d表达神经细胞标记蛋白和mRNA;对照组不表达神经细胞标记蛋白和mRNA。证实黄芩甙在体外可诱导成年大鼠骨髓基质细胞分化为神经样细胞。
BACKGROUND: Experiments have shown that bone marrow stromal cells have great potential to differentiate into bone, neural stem cells and hematopoietic stem cells, and baicalin has the effect of inducing cell differentiation. Objective: To explore the possibility that baicalin induces the differentiation of bone marrow stromal cells into neural stem cells in vitro. Methods: BMSCs were isolated and cultured from Wistar rats. The isolated BMSCs were divided into experimental group and control group. The control group received no intervention. The experimental group was induced with baicalin (350 ~ 400μmol / L) The same culture environment. After 6 days of continuous induction, well-growing cells were selected and tested by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). RESULTS AND CONCLUSION: After 7 days of baicalin induction, bone marrow stromal cells formed more typical neuronal morphology. BMSCs did not express neuronal marker protein and protein before induction; neural cell marker protein and mRNA were expressed 6 d after induction; neural cell marker protein and mRNA were not expressed in control group. Confirmed that baicalin in vitro can induce adult rat bone marrow stromal cells differentiate into neuron-like cells.