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研究了25℃、30℃和35℃三个出芝温度条件下,利用人工气候箱袋式栽培灵芝,其基质不同时期-部位的物质含量(总糖、还原糖、水溶性蛋白和游离氨基酸)与木质素酶系酶(漆酶和锰过氧化物酶)、纤维素酶系酶(羧甲基纤维素酶和滤纸酶)和酸性蛋白酶活性变化规律。结果显示,在25℃时,灵芝能够正常出芝,温度高于30℃以后子实体分化受到抑制;且在25℃条件下,不同时期-部位基质中总糖和可溶性蛋白含量低于其它2个温度处理组,而还原糖和游离氨基酸含量高于其它2个温度处理组;同时,不同时期-部位基质中木质素酶系和纤维素酶系酶活力低于其它2个温度处理组,但酸性蛋白酶活力高于另外其它2个温度处理组。提示袋料灵芝栽培时出芝温度控制在25℃左右利于其子实体生长发育。
In this study, Ganoderma lucidum was cultivated in a box with artificial climate at 25 ℃, 30 ℃ and 35 ℃. The content of substance (total sugar, reducing sugar, water-soluble protein and free amino acid) And ligninase enzymes (laccase and manganese peroxidase), cellulase enzymes (carboxymethyl cellulose and filter paper enzymes) and acid protease activity changes. The results showed that at 25 ℃, Ganoderma lucidum could produce normal Zhizhi, and the fruiting body differentiation was inhibited when the temperature was higher than 30 ℃. At 25 ℃, the contents of total sugar and soluble protein in the matrix were lower than the other two Temperature treatment group, while the contents of reducing sugar and free amino acid were higher than those of the other two temperature treatment groups. At the same time, the activities of ligninase and cellulase enzymes in different parts of the matrix were lower than those of the other two temperature treatment groups, but the acidity Protease activity was higher than the other two temperature treatment groups. Suggest bags Ganoderma Ganoderma lucidum cultivation temperature of about 25 ℃ conducive to its fruiting body growth and development.