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玉米成熟子粒含有较多的多糖、蛋白质和脂类,传统的RNA提取方法难以提取到高质量的RNA。以授粉后28 d灌浆后期的玉米子粒为试材,以授粉后7 d的玉米子粒为对照,对目前国内外常用的Trizol法、Biozol法、RNAiso Plus法、三种硅胶柱商业试剂盒总RNA提取方法以及本研究建立的改良提取方法进行比较。结果表明,以授粉后7 d的玉米子粒为材料时,各种方法都能获得较好提取效果;以灌浆后期接近成熟玉米子粒为试材时,采用本研究改良或新建立的提取法,可以大幅降低提取成本,快速提取高质量和高得率的RNA。
The maize mature grains contain more polysaccharides, proteins and lipids, and the traditional RNA extraction method is difficult to extract high quality RNA. At 28 d after pollination, the maize seeds at the late filling stage were used as experimental materials. The maize seeds 7 d after pollination were used as control. The Trizol, Biozol, RNAiso Plus and three common silica gel commercial kits Extraction method and the improved extraction method established in this study for comparison. The results showed that all the methods could get good results when the maize seeds on the 7th day after pollination were used as materials. When the near mature maize seeds were used as materials in the late filling stage, the improved or newly established extraction method could be used Dramatically reduce the cost of extraction, rapid extraction of high quality and high yield of RNA.