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通过拟南芥、水稻和甜高粱BIC1、BIC2氨基酸同源序列比对,发现blue-light inhibitor of cryptochromes 1(BIC1)和blue-light inhibitor of cryptochromes 2(BIC2)有很高的相似性,尤其在与Cryptochromes相互作用的CID结构域。并通过12个不同物种BICs蛋白的进化分析发现,单子叶、双子叶和低等植物的BIC1和BIC2有明显的分支,而甜高粱BIC1和BIC2分别与玉米的BIC1和水稻的BIC2亲缘关系最近,这些结果暗示着BICs在单子叶和双子叶植物间进化中可能发生了分歧但功能可能是保守的。通过Sb BIC1、Sb BIC2与拟南芥CRY1、CRY2及甜高粱CRY1a、CRY1b的酵母双杂交分析发现,Sb BIC2与拟南芥CRY1、CRY2及甜高粱CRY1a、CRY1b都能发生蓝光依赖的相互作用,这个结果说明在甜高粱中Sb BIC2可能参与了CRYs调控的光形态建成。
Through the alignment of amino acid sequences of BIC1 and BIC2 in Arabidopsis thaliana, rice and sweet sorghum, there was a high similarity between blue-light inhibitor of cryptochromes 1 (BIC1) and blue-light inhibitor of cryptochromes 2 (BIC2) CID domain that interacts with Cryptochromes. The evolutionary analysis of BICs in 12 different species showed that BIC1 and BIC2 in monocotyledon, dicotyledonous and lower plants had obvious branching, whereas sweet sorghum BIC1 and BIC2 had the closest genetic relationship with BIC1 in maize and BIC2 in rice, respectively. These results suggest that BICs may diverge in the evolution between monocotyledon and dicotyledonous plants but may function conservatively. The yeast two-hybrid analysis of Sb BIC1, Sb BIC2 and Arabidopsis CRY1, CRY2 and sweet sorghum CRY1a and CRY1b revealed that there was a blue-light-dependent interaction between Sb BIC2 and CRY1, CRY2 and CRY1a and CRY1b in Arabidopsis thaliana, This result suggests that Sb BIC2 may be involved in the regulation of CRYs in sweet sorghum.