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目的:探讨TIMP-3基因甲基化与结直肠癌临床病理指标和转移复发的关系。方法:采用巢式甲基化特异性PCR技术(NMSP法)检测100例结直肠癌组织和100例癌旁非癌组织TIMP-3基因甲基化;采用RT-PCR 检测100例结直肠癌组织和100例癌旁非癌组织TIMP-3 MRNA的表达。结果:肿瘤组织TIMP-3 MRNA的表达阳性率为64％,肿瘤组织TIMP-3 MRNA的表达率明显低于癌旁非癌组织(P<0．01);TIMP-3 MRNA的表达率无淋巴结转移组(34/42)高于淋巴结转移组(30/58)(P<0．01),甲基化阳性率DUKE’S C+D期伴淋巴结转移组明显高于 DUKE’S A+B期不伴淋巴结转移组(P<0．05)。结肠近端、分化程度差的结直肠癌组织甲基化阳性率明显高于远端直肠和分化程度高者(P<0．05)。结论:TIMP-3基因甲基化容易发生在结肠近端、DUKE’S C、D期、伴淋巴结转移、细胞分化差和浸润型结直肠癌患者。
Objective: To investigate the relationship between methylation of TIMP-3 gene and clinicopathological features and metastasis and recurrence of colorectal cancer. Methods: Methylation of TIMP-3 gene in 100 cases of colorectal cancer tissues and 100 cases of non-cancerous non-cancerous tissues was detected by nested methylation-specific PCR (NMSP) method. 100 cases of colorectal cancer tissues And 100 cases of adjacent non-cancerous tissue TIMP-3 MRNA expression. Results: The positive expression rate of TIMP-3 MRNA in tumor tissue was 64%, the expression rate of TIMP-3 MRNA in tumor tissue was significantly lower than that in non-cancerous non-cancerous tissue (P <0.01); The expression rate of TIMP-3 MRNA was no lymph node Metastasis group (34/42) was higher than that of lymph node metastasis group (30/58) (P <0.01). Methylation positive rate DUKE’S C + D stage with lymph node metastasis was significantly higher than DUKE’S A + B stage without lymph node metastasis Transfer group (P <0.05). The positive rate of methylation in the proximal colon and poorly differentiated colorectal cancer tissues was significantly higher than that in the distal rectum and the higher degree of differentiation (P <0.05). CONCLUSIONS: TIMP-3 gene methylation is prone to occur in the proximal colon, DUKE’S C, D stage, with lymph node metastasis, poorly differentiated cells and invasive colorectal cancer.