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目的通过养胃颗粒灌胃给药对小鼠的急性毒性实验,为临床应用提供科学依据。方法根据中药、天然药物急性毒性实验技术指导原则,将小鼠40只按体质量随机分为养胃颗粒组和空白对照组,禁食不禁水12 h后,养胃颗粒组将养胃颗粒以最大浓度(2.1 g·ml-1),最大体积[0.4 ml·(10 g)-1]灌胃给药,空白对照组以等容积的蒸馏水灌胃给药,连续14d观察小鼠体质量,行为活动以及死亡情况。14 d后处死所有小鼠,观察小鼠心、肝、肾、脑等脏器是否正常。结果养胃颗粒组小鼠无1例死亡,与对照组比较,养胃颗粒组小鼠的体质量变化和进食量无显著差异,行为活动无异常。尸解后未见养胃颗粒组小鼠的脏器异常。结论养胃颗粒对小鼠急性毒性甚低,在规定剂量下服用安全可靠。
Objective To study the acute toxicity of Yangwei granule in mice by intragastric administration, and provide scientific basis for clinical application. Methods According to the guidelines of experimental techniques of acute toxicity of traditional Chinese medicine and natural medicine, 40 mice were randomly divided into Yangwei granule group and blank control group according to their body weight. After fasting for 12 h, Yangwei granule group The rats in the blank control group were intragastrically administrated with equal volume of distilled water for 14 days. The body weight, Behavioral activity and death. After 14 d, all mice were sacrificed to observe whether the mice heart, liver, kidney, brain and other organs were normal. Results Yangwei granule group mice no one death, compared with the control group, Yangwei granule group mice body weight changes and food intake was no significant difference in behavior activity was normal. No autotrophic organs group mice organ abnormalities. Conclusion Yangwei granule has very low acute toxicity to mice and is safe and reliable under the prescribed dosage.