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目的：阐明γ干扰素抑制血管内膜增生和防治再狭窄的体内机制。 方法：建立兔再狭窄血管模型。将６０只新西兰纯种兔分为实验组（ｎ＝ ３０）术前１ 天给予重组γ干扰素和对照组（ｎ＝３０）术前不给干扰素。每组分别于术后１周、２ 周和４周处死动物，每次１０只。动态观察给予重组γ干扰素对损伤后不同时期内膜血管平滑肌细胞（ＶＳＭＣ）原位表达ｃ－ｍ ｙｃ信使核糖核酸（ｍＲＮＡ）和增殖细胞核抗原的影响。 结果：γ干扰素显著抑制１ 周和４周时内膜ＶＳＭＣ增殖细胞核抗原的表达，抑制率分别为８８．５０％ 和５８．８９％ ；显著抑制新生内膜ＶＳＭＣｃ－ｍ ｙｃｍ ＲＮＡ的表达，抑制率分别为１周９１．４５％ 、２ 周９３．６６％ 和４ 周５２．９２％ 。 结论：γ干扰素抑制内膜ＶＳＭＣ增殖和再狭窄伴ｃ－ｍ ｙｃ癌基因表达的下调，为防治再狭窄提供了一条途径
OBJECTIVE: To elucidate the mechanism of interferon gamma in inhibiting intimal hyperplasia and preventing restenosis. Methods: Rabbit restenosis vascular model was established. Sixty New Zealand purebred rabbits were divided into experimental group (n = 30), given recombinant interferon gamma and control group (n = 30) one day before surgery, and no interferon was given before operation. Animals in each group were sacrificed at 1 week, 2 weeks and 4 weeks after operation, 10 animals each. The effect of recombinant interferon gamma on in situ expression of c-m yc messenger RNA (mRNA) and proliferating cell nuclear antigen (PCNA) in VSMCs was observed dynamically. RESULTS: Interferon gamma significantly inhibited the expression of proliferating cell nuclear antigen (PCNA) in VSMCs at 1 week and 4 weeks, with inhibitory rates of 88.50% and 58.89%, respectively. It significantly inhibited the expression of neointimal VSMCc-m ycm RNA and inhibited Rates were 91.45% for 1 week, 93.66% for 2 weeks and 52.92% for 4 weeks, respectively. CONCLUSION: IFN-γ inhibits the down-regulation of c-Myc oncogene expression in VSMCs with proliferation and restenosis in intima, which provides a way to prevent restenosis