论文部分内容阅读
Novel promoters that confer root-specific expression would be useful for engineering resistance against problems of nutrient and water absorption by roots. In this study, the reverse transcriptase polymerase chain reaction was used to identify seven genes with root-specific expression in rice. The isolation and characterization of upstream promoter regions of five selected genes rice root-specific promoter(r RSP) 1 to 5(r RSP1-r RSP5) and A2P(the promoter of Os Act2) revealed that r RSP1, r RSP3, and r RSP5 are particularly important with respect to root-specific activities. Furthermore, r RSP1, r RSP3, and r RSP5 were observed to make different contributions to root activities in various species. These three promoters could be used for root-specific enhancement of target gene(s).
Novel promoters that confer root-specific expression would be useful for engineering resistance against problems of nutrient and water absorption by roots. In this study, the reverse transcriptase polymerase chain reaction was used to identify seven genes with root-specific expression in rice. The isolation and characterization of upstream promoter regions of five selected genes rice root-specific promoter (r RSP) 1 to 5 (r RSP1-r RSP5) and A2P (the promoter of Os Act2) revealed that r RSP1, r RSP3, and r RSP5 are particularly, with respect to root-specific activities. These three promoters could be used for root-specific enhancement of target gene (s ).