论文部分内容阅读
【目的】研究双精氨酸运输系统(Tat)与肠侵袭性大肠杆菌(EIEC)生物学特性之间的关系。【方法】通过同源重组的方法,构建EIEC的tatABC基因缺失菌株以及互补菌株,并探索其对细菌形态、底物转运功能以及对HeLa细胞和豚鼠角膜侵袭力的影响。【结果】TatABC基因缺失株细菌形态变化明显,底物转运功能丧失,细菌侵袭力也显著减弱(缺失株侵入HeLa细胞数量明显减少,致豚鼠角膜病变能力明显减弱),而互补菌株在上述方面较接近野生株。【结论】EIEC的Tat蛋白运输系统与EIEC的生物学特性有密切关系。
【Objective】 To investigate the relationship between the bi-arginine transport system (Tat) and the biological characteristics of intestinal invasive Escherichia coli (EIEC). 【Method】 The tatABC gene deletion strains and complementary strains of EIEC were constructed by homologous recombination, and their effects on bacterial morphology, substrate transport function and invasiveness of HeLa cells and cornea of guinea pigs were explored. 【Result】 The results showed that the bacterial morphology of TatABC gene deletion was obviously changed, the transport function of the substrate was abolished, and the invasiveness of bacteria was significantly weakened (the number of invaded HeLa cells was significantly reduced and the ability of corneal lesion of guinea pigs was obviously weakened), while the complementary strains were closer to the above Wild strain. 【Conclusion】 The EI protein transport system is closely related to the biological characteristics of EIEC.