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利用毛新杨×毛白杨的杂交群体构建了毛新杨×毛白杨的AFLP分子遗传图谱,这个群体对杨树溃疡病的抗性是正态分布。本实验使用AFLP分子标记技术对这一构图群体进行了分析,共选用了19对PstI/MseI引物和4对EcoRI/MseI引物,得到标记662个,其中3∶1标记占43 2%,异倍标记占14 5%,偏分离标记(P<0 05)占48 8%。在双亲整合的图谱中,239个标记形成22个含4个以上标记的连锁群,总图距4418cM,标记间的平均图距是18 5cM。图中还有8个重复位点和7个只与连锁群中的某一个标记连锁,但却无法加入这一连锁群的标记,这可能是由染色体的同源性造成的。此外还得到15个属于双亲共有的只有2～3个标记的小连锁群,13个父本独有的连锁群和21个母本独有的连锁群。
The AFLP molecular genetic map of MaoXinYang × Populus tomentosa was constructed using the hybrid population of MaoXinYang × Populus × euphratica, and the population resistance to poplar canker disease was normal distribution. In this experiment, AFLP molecular marker technique was used to analyze this population. A total of 662 markers were selected from 19 pairs of PstI / MseI primers and 4 pairs of EcoRI / Msel primers, of which 3: 1 was 43.2% Marked 14 5%, partial separation mark (P <0 05) accounted for 48 8%. In the parents integrated map, 239 markers formed 22 linkage groups with more than 4 markers with a total map distance of 4418cM and the average distance between markers was 18 5cM. There are also eight repeat sites and seven markers that are only linked to one of the linkage groups but can not join the linkage group, probably due to the homology of the chromosomes. In addition, there are 15 small linkage groups shared by parents, only two or three markers, 13 unique linkage groups and 21 maternal linkage groups.