论文部分内容阅读
目的 探讨基因重组转化生长因子 α 绿脓杆菌外毒素融合蛋白 (TGFα PE40 ,简称TP40 )对人低分化胃癌细胞生长的导向抑制作用。方法 用免疫组化LSAB法检测人低分化胃癌MKN 45细胞表皮生长因子受体 (EGFR)的表达 ,用结晶柴染色法和3 H 亮氨酸掺入法检测TP40对MNK 45细胞生长及蛋白质合成的抑制 ,用过量EGF竞争拮抗TP40的细胞毒作用。结果 MKN 45细胞免疫组化显示出大量棕黄色的EGFR阳性染色。TP40浓度为 1～ 10 0ng/ml时 ,对MKN 45细胞长生及蛋白质合成呈剂量依赖性抑制。过量EGF能完全拮抗TP40的细胞毒作用。结论 人低分化胃癌MKN 45细胞能高水平地表达EGFR ,重组TP40对MKN 45细胞生长具有较强的抑制作用 ,作用部位为细胞的EGFR
Objective To investigate the inhibitory effect of recombinant human transforming growth factor-α (Pseudomonas aeruginosa exotoxin fusion protein, TP40) on human poorly differentiated gastric cancer cells. Methods The expression of epidermal growth factor receptor (EGFR) in human poorly differentiated gastric cancer MKN 45 cells was detected by immunohistochemical staining with LSAB method. The growth and protein synthesis of MNK 45 cells by TP40 and 3 H leucine incorporation assay Inhibition of EGF, EGF with excessive competition antagonistic TP40 cytotoxicity. Results Immunohistochemistry of MKN 45 cells showed a large number of brown-positive EGFR-positive staining. TP40 concentration of 1 ~ 10 0ng / ml, MKN 45 cell growth and protein synthesis in a dose-dependent manner. Excess EGF completely antagonized the cytotoxic effect of TP40. Conclusion Human gastric cancer MKN 45 cells express EGFR at a high level. Recombinant TP40 can inhibit the growth of MKN 45 cells in a dose-dependent manner. The site of action is EGFR