论文部分内容阅读
目的初步探讨HBV感染者血清中的乙肝大蛋白(HBV-LP)检测的临床意义,并对其与Pre S1抗原、HBV DNA之间的关系进行分析。方法采用酶联免疫吸附试验(ELISA)方法检测192例HBV感染者血清中的HBV-LP,Pre S1抗原及HBV分子标志物(HBV M),并用荧光定量PCR方法检测HBV DNA。结果 192例HBV感染者血清HBVLP、Pre S1抗原、HBV DNA及HBe Ag阳性率分别为67.18%、54.17%、52.60%和9.38%,HBV-LP阳性率高于Pre S1抗原阳性率(χ~2=6.82,P<0.05)、HBV DNA检测阳性率(χ~2=8.50,P<0.05)及HBe Ag阳性率(χ~2=135.80,P<0.05);HBVLP含量(A值)与HBV DNA拷贝数的对数值呈正相关关系(r=0.798,P<0.05)。结论血清中HBV-LP的含量与HBV DNA的拷贝数相关性较好,HBV-LP能准确的反映乙肝病毒复制情况,是HBe Ag、Pre S1抗原的重要补充。
Objective To investigate the clinical significance of detection of hepatitis B large protein (HBV-LP) in serum of patients with HBV infection and to analyze its relationship with Pre S1 antigen and HBV DNA. Methods Serum HBV-LP, Pre S1 antigen and HBV molecular marker (HBV M) were detected by enzyme-linked immunosorbent assay (ELISA) in 192 HBV infected patients. HBV DNA was detected by real-time PCR. Results The positive rates of serum HBVLP, Pre S1, HBV DNA and HBeAg in 192 HBV infected patients were 67.18%, 54.17%, 52.60% and 9.38%, respectively. The positive rate of HBV-LP was higher than that of Pre S1 antigen (χ ~ 2 = 6.82, P <0.05), the positive rate of HBV DNA test (χ ~ 2 = 8.50, P <0.05) and the positive rate of HBe Ag (χ ~ 2 = 135.80, P < There was a positive correlation between copy number logarithm (r = 0.798, P <0.05). Conclusion HBV-LP in serum has a good correlation with the copy number of HBV DNA. HBV-LP can accurately reflect the replication of hepatitis B virus and is an important supplement to HBe Ag and Pre S1 antigens.