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目的:分离获得经确证的竹黄的无性型菌种。方法:从竹黄的子实体中分别通过组织分离和活子囊孢子分离获得不同的子实体分离株。通过培养性状,微观形态特征观察和5.8S-ITS rDNA序列的分析对分离获得的菌株进行确证。结果:应用不同的分离方法分离到的菌株GZDXIFR-171,GZDXIFR-181,它们具有相同的菌落形态特征。用ITS1-5.8S rDNA-ITS2的通用引物,经PCR扩增、测序得到GZDXIFR-171,GZDXIFR-181的5.8S-ITS rDNA序列。将这个序列经NCBI Blast进行核苷酸比对发现GZDXIFR-171,GZDXIFR-181菌株与NCBI中已登录竹黄序列的相似性均为99%~100%。结论:分离获得的GZDXIFR-171,GZDXIFR-181菌株为竹黄菌Shiraia bambusicola的真正无性型。
OBJECTIVE: To isolate the vegetative strains of bamboo yellow that have been confirmed. Methods: Different fruiting body isolates were obtained from the fruiting bodies of bamboo yellow, through tissue separation and live ascospores isolation. The isolated strains were confirmed by culturing traits, microscopic morphological characteristics and 5.8S-ITS rDNA sequence analysis. RESULTS: The strains GZDXIFR-171 and GZDXIFR-181 were isolated using different isolation methods. They had the same colony morphological characteristics. Using the universal primers of ITS1-5.8S rDNA-ITS2, the 5.8S-ITS rDNA sequences of GZDXIFR-171 and GZDXIFR-181 were obtained by PCR amplification and sequencing. Nucleotide alignment of this sequence by NCBI Blast revealed that the similarity of GZDXIFR-171 and GZDXIFR-181 strains with the registered bamboo yellow sequences in NCBI was 99% to 100%. Conclusion: The isolated GZDXIFR-171 and GZDXIFR-181 strains are the true anamorph of Shiraia bambusicola.