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目的 探讨肺癌K ras基因突变与呼吸道合胞病毒 (RSV)感染的关系。方法 应用反转录聚合酶链反应 (RT PCR)结合单链构象多态性分析 (SSCP)免疫组化和ELISA方法检测肺癌组织中RSV、K ras基因和血清sIgM抗体。结果 ①RT PCR显示17.9% ( 7/3 9)的肺癌标本中存在有RSV基因序列。②RSVRT PCR阳性的肺癌组织冰冻切片免疫组化显示RSV抗原在细胞浆呈弥漫性着色。③不经反转录反应直接作PCR扩增 ,则RSV阳性的标本不能观察到RSV特异的电泳区带。④SSCP电泳示 2 5 .6% ( 10 /3 9)的肺癌组织标本中K ras基因的第 12位密码子有突变。⑤肺癌组织RSV基因的检出与K ras基因突变结果经配对计数资料McNemar检验分析 ,χ2 =0 .3 6,P >0 .0 5。结论 肺癌组织内有RSV感染 ,可能和K ras基因的突变有关。
Objective To investigate the relationship between K ras gene mutation and respiratory syncytial virus (RSV) infection in lung cancer. Methods Reverse transcription-polymerase chain reaction (RT PCR) combined with single-strand conformation polymorphism analysis (SSCP) immunohistochemistry and ELISA were used to detect RSV and Kras genes and serum sIgM antibodies in lung cancer tissues. Results 1 The RT-PCR showed that there were RSV gene sequences in 17.9% (7/39) lung cancer specimens. 2RSVRT PCR positive frozen section of lung cancer tissue immunohistochemistry showed diffuse staining of RSV antigen in the cytoplasm. 3 RSV-specific electrophoretic bands could not be observed in RSV-positive specimens without direct reverse transcription reaction for PCR amplification. 4 SSCP electrophoresis showed mutations in the 12th codon of the K ras gene in 25.6 % (10/39) lung cancer tissue samples. 5 The detection of RSV gene and K ras gene mutation in lung cancer tissues were analyzed by paired-count data McNemar analysis, χ 2 =0.36, P > 0.05. Conclusion There are RSV infections in lung cancer tissues, which may be related to mutations in the K ras gene.