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目的建立同时分析醒酒护肝产品中儿茶素、表儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、二氢杨梅素、甘草酸和甘草次酸等8种化合物的高效液相色谱法,并与实验室已建立的毛细管电泳法进行方法学比较。方法样品经甲醇-水(4∶1,V/V)超声提取30 min,10 000 r/min离心10 min,取上清液过滤膜后液相色谱进样,采用C_(18)柱(5μm×250 mm×4.6 mm)进行梯度洗脱,流速为0.8 mL/min,色谱柱温度为30℃,采用可变波长检测器在210 nm处对儿茶素类及二氢杨梅素进行检测,250 nm处对甘草酸及甘草次酸进行检测。结果在优化的条件下,8种组分在各自的线性范围内相关系数(r)≥0.9996,检出限和定量限分别为0.07~1.25μg/g(S/N=3)和0.22~4.18μg/g(S/N=10),日内精密度和日间精密度分别为0.26%~1.95%和1.17%~3.89%,加标回收率为86.15%~98.61%。结论该高效液相色谱法灵敏度高、重复性好,可用于醒酒护肝产品的质量控制。
OBJECTIVE To establish a method for simultaneous determination of catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, dihydromyricetin, glycyrrhizin and licorice Acid and other 8 kinds of compounds by high performance liquid chromatography and laboratory established capillary electrophoresis methodological comparison. Methods The samples were ultrasonically extracted with methanol - water (4: 1, V / V) for 30 min and centrifuged at 10,000 r / min for 10 min. The supernatant was filtered through a liquid chromatographic system and loaded on a C 18 column × 250 mm × 4.6 mm) at a flow rate of 0.8 mL / min and a column temperature of 30 ° C. Catechins and dihydromyricetin were detected at 210 nm with a variable wavelength detector. 250 nm at glycyrrhizic acid and glycyrrhetinic acid were detected. Results Under the optimal conditions, the correlation coefficient (r) of the eight components in the linear range was 0.99996. The limits of detection and quantification were 0.07-1.25 μg / g (S / N = 3) and 0.22-4.18 μg / g (S / N = 10). The intra-day precision and intra-day precision were 0.26% -1.95% and 1.17% -3.89% respectively. The recoveries were in the range of 86.15% -98.61%. Conclusion The HPLC method has high sensitivity and repeatability and can be used for quality control of sober liver products.