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目的建立免疫印迹法(WB)检测抗毒蕈碱受体3(M3受体)抗体的方法,探讨该抗体对干燥综合征(SS)诊断的意义。方法建立WB测定抗M3受体抗体的方法,检测80例SS患者、80例其他风湿性疾病及40名正常对照者血清中的抗M3受体抗体,并分析该抗体与SS的临床及实验室指标的关系。结果抗M3受体抗体在80例SS患者中的阳性率为83.8%(67/80),而在系统性红斑狼疮(SLE)及正常人中分别为5.0%(2/40)和2.5%(1/40),类风湿关节炎(RA)中无1例阳性。抗M3受体抗体对SS的敏感性和特异性分别为83.8%和97.5%,阳性预测值为95.7%,阴性预测值为90.0%。抗M3受体抗体在抗SSA抗体、抗SSB抗体、琢-胞衬蛋白抗体阴性的SS患者血清中的阳性率分别为80.0%、82.5%及81.2%。该抗体在抗SSA抗体、抗SSB抗体及琢-胞衬蛋白抗体阳性的SS患者血清中的阳性率分别为87.5%、88.2%及85.4%。抗M3受体抗体阳性的SS患者与该抗体阴性的SS患者在口干、眼干、脏器受累的发生率,IgG、IgA、血沉(ESR)升高及抗核抗体(ANA)、类风湿因子(RF)阳性率方面无统计学意义。结论利用sf9细胞表达的重组M3受体蛋白可以检测SS患者血清中的抗M3受体抗体。该抗体是一种对SS诊断较为特异的标记性抗体之一。
Objective To establish a method for the detection of anti-muscarinic receptor 3 (M3 receptor) antibodies by Western blotting and explore the significance of this antibody in the diagnosis of Sjogren’s syndrome (SS). Methods The anti-M3 receptor antibody was determined by WB method. The anti-M3 receptor antibodies in serum of 80 patients with SS, 80 other rheumatic diseases and 40 normal controls were detected, and the relationship between the antibody and SS clinical and laboratory The relationship between indicators. Results The positive rate of anti-M3 receptor antibody was 83.8% (67/80) in 80 patients with SS and 5.0% (2/40) and 2.5% (2/40) in patients with systemic lupus erythematosus (SLE) 1/40), and no one was positive in rheumatoid arthritis (RA). The sensitivity and specificity of anti-M3 receptor antibodies to SS were 83.8% and 97.5%, respectively, with a positive predictive value of 95.7% and a negative predictive value of 90.0%. The positive rates of anti-M3 receptor antibodies in sera of SS patients with anti-SSA antibody, anti-SSB antibody, and Î ± -reduin antibody were 80.0%, 82.5% and 81.2%, respectively. The positive rates of this antibody in sera of patients with anti-SSA antibody, anti-SSB antibody and seminal-integrin antibody were 87.5%, 88.2% and 85.4%, respectively. The incidence of dry mouth, dry eye and organ involvement, IgG, IgA, ESR and anti-nuclear antibody (ANA) in rheumatoid arthritis patients with anti-M3 receptor antibody- No significant difference was found in RF positive rate. Conclusion The recombinant M3 receptor protein expressed in sf9 cells can detect anti-M3 receptor antibodies in serum of patients with SS. This antibody is one of the more specific markers for the diagnosis of SS.