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优降糖是有代表性的第二代磺脲药,为了研究它在体内的代谢变化,我们建立了血浆优降糖浓度高压液相色谱测定法(HPLC法),并进行了正常人口服优降糖的药代动力学研究。测定血浆优降糖HPLC法:内标法定量测定,座标系呈高度直线相关,Y=0.99999,线性范围25～400ng/ml。回归方程:X=0.3918Y～0.0017(X=浓度比,Y=相应校正因子)。高、中、低浓度的优降糖标准血浆样品的批间、批内变异系数均<11％。平均回收率:高浓度=101.4±7.6％,中浓度=103.3±11.4％,低浓度=98.8±8.0％。
Glibenclamide is a representative second-generation sulfonylurea. In order to investigate its metabolic changes in vivo, we established a HPLC-HPLC method for the determination of plasma hypoglycemic concentration and performed oral administration Pharmacokinetics of hypoglycemic agents. Determination of plasma hypoglycemic HPLC method: internal standard quantitative determination of the coordinate system was highly linear correlation, Y = 0.99999, the linear range of 25 ~ 400ng / ml. Regression equation: X = 0.3918Y ~ 0.0017 (X = concentration ratio, Y = corresponding correction factor). High, medium and low concentrations of glyburide standard plasma samples batch, intra-assay CV <11%. Average recovery: high concentration = 101.4 ± 7.6%, medium concentration = 103.3 ± 11.4%, low concentration = 98.8 ± 8.0%.