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本文探讨了不同分子量普洱茶茶褐素对高脂血症SD大鼠的治疗作用。通过45 d的治疗,饲喂TB1和RTB的大鼠血清TCTG和LDL均显著低于高脂模型组(p<0.01),HDL显著高于高脂模型组(p<0.01),且TG与HDL与正常对照组无显著性差异。TB1和RTB对肝脏组织中HSL酶活性影响不显著,但在附睾组织和肠系膜组织中HSL活性极显著高于高脂模型组(p<0.01),与正常对照组差异不显著(p>0.01)。同时,TB1和RTB可显著抑制高脂血症大鼠脂肪组织ACC酶活性(p<0.01)和FAS酶活性(p<0.05)。说明TB1和RTB可通过提高HSL活性来加速脂质代谢,同时通过抑制ACC和FAS活性来减少脂肪酸合成。Western Blot检测发现,TB1和RTB有上调HSL蛋白表达、下调ACC1蛋白表达的作用。肝脏病理切片显示,TB1和RTB能有效减缓和降低高脂血症大鼠肝脏脂肪变性的速度和程度。研究结果表明,TB1是RTB的主要活性成分,对高脂血症大鼠有一定的治疗作用。
This article discusses the different molecular weight of Pu-erh tea-browning on the treatment of hyperlipidemic SD rats. After 45 days treatment, TCTG and LDL in serum of rats fed TB1 and RTB were significantly lower than those in high fat model group (p <0.01), HDL was significantly higher than that in high fat model group (p <0.01), and TG and HDL No significant difference with the normal control group. The activity of HSL in liver tissue was not significantly affected by TB1 and RTB, but HSL activity in epididymal and mesenteric tissue was significantly higher than that in high fat model group (p <0.01), but not significantly different from that in normal control group (p> 0.01) . At the same time, TB1 and RTB significantly inhibited ACC activity (P <0.01) and FAS activity (P <0.05) in adipose tissue of hyperlipidemic rats. It is suggested that TB1 and RTB can accelerate lipid metabolism by increasing HSL activity while reducing fatty acid synthesis by inhibiting ACC and FAS activity. Western Blot detection, TB1 and RTB have up-regulated HSL protein expression, down-regulation of ACC1 protein expression. Liver biopsy showed that TB1 and RTB can effectively reduce and reduce the rate and extent of hepatic steatosis in hyperlipidemic rats. The results showed that TB1 is the main active ingredient of RTB, and has a certain therapeutic effect on hyperlipidemic rats.