球虫的生活史研究中,对最早的内生发育期,特别是对子孢子的定位常有困难。过去所用的染色方法,由于对其他细胞成分染成相同颜色,而且虫体小,常单个深藏于肠粘膜隐窝中,子孢子难于发现,往往仍需用高倍镜观察。子孢子的特征是具有一个折光球。 In the life history of coccidia, it is often difficult to locate the earliest endometriosis, especially sporozoites. The dyeing method used in the past, due to the same color of the other cell components stained, and the small insect body, often single hidden in the crypt of the intestinal mucosa, sporozoites difficult to find, often still need to use high magnification observation. Sporozoites are characterized by a refractive sphere.