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在组织培养过程中对喜树叶片外植体进行解剖学观察。发现在组织培养条件下,喜树叶片在培养基的酸性环境中细胞壁呈现模糊微弱降解、明显降解和完全降解直至消失的解剖学特征。在同样的组培条件和相同的时间内,同一喜树叶片不同部位出现细胞壁程度不同的降解和消失现象,可能是喜树叶片因上表皮凸凹不平进而导致其不同部位与酸性培养基接触的程度不同,因而使培养基中的酸性物质对喜树叶片上表皮的不同部位影响出现异质化。本文对培养基中的酸性成分对喜树叶片细胞壁降解的影响有了进一步的认识和理解。
Anatomical observation of explants of Camptotheca acuminata leaves during tissue culture. It was found that in the tissue culture conditions, the leaves of the leaves of Camptotheca acuminata showed a faintly degraded, significantly degraded and completely degraded cell wall in the acidic environment of the culture medium until disappearing anatomical features. Under the same conditions of tissue culture and same time, the degradation and disappearance of cell wall in different parts of the same Camptotheca acuminata leaves may be caused by the unevenness of the upper epidermis which leads to the contact of different parts with acid medium Different, so that the acidic substances in the medium on the different parts of the epidermis on the leaves of the tree heterogeneity. In this paper, we further understand and understand the influence of acidic components in culture medium on the degradation of cell wall of Camptotheca acuminata leaves.