论文部分内容阅读
为研究套袋对不知火果实品质和类胡萝卜素代谢的影响,选用生产上常用的外黄内黑双层纸袋对不知火果实进行套袋处理,以不套袋为对照.果实品质相关指标测定结果表明:不知火果实套袋后果实单质量增加,可滴定酸和可溶性固形物质量分数降低,Vc质量浓度、出汁率和可食率变化不大.利用HPLC检测对套袋和未套袋果实果皮紫黄质、9-z-紫黄质、β-隐黄质、a-胡萝卜素、β-胡萝卜素和八氢番茄红素质量比进行了测定,发现套袋果实果皮中这些类胡萝卜素类物质质量比均低于未套袋果实.利用实时荧光定量PCR研究了套袋对果皮CRTISO,LCYb,LCYe,PDS,PSY,ZDS和ZEP基因表达的影响,发现:套袋不知火果皮中这些基因的表达均显著低于未套袋果实.该研究结果表明:套袋可提高不知火外观品质,对不知火果实质量的增加具有一定的促进作用,同时,套袋使不知火果实风味下降.此外,该研究发现套袋还显著降低了不知火果皮类胡萝卜素质量比,这可能是由于果实套袋使得阳光无法透过,进而使得类胡萝卜素代谢相关基因的表达降低引起的.
In order to study the effect of bagging on the quality of fire fruits and the metabolism of carotenoid, the outer double yellow paper bag, which is commonly used in the production, was used to bag the bagged baguette without any bagging, and the results of fruit quality index : I do not know the fruit after bagged fruit single quality increase, titratable acid and soluble solids content decreased, Vc concentration, juice yield and edible rate changed little.Using HPLC detection bagged and un bagged fruit peel purple Quality of 9-z-violaxanthin, β-cryptoxanthin, a-carotene, β-carotene and phytoene were measured and found that the quality of these carotenoids in bagged fruit peels The results showed that the expression of CRTISO, LCYb, LCYe, PDS, PSY, ZDS and ZEP in the pericarp of bagged peanut was studied by real-time fluorescence quantitative PCR Significantly lower than the unaggregated fruit.The results showed that: Bagging can improve the quality of the fire ignorant of the appearance, I do not know the increase of the quality of fire fruit has a certain role in promoting, at the same time, bagging so I do not know the flavor of fire fruit declines.In addition, the research It was found that bagging also significantly reduced the fire peel carotenoid mass ratio, which may be due to the bagging makes the sun can not be through, so that the expression of carotenoid metabolism-related genes decreased.