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【目的】对新疆自育的新海系列品种进行遗传多样性研究。【方法】利用SRAP和RGA标记,检测出多态性位点;利用NTSYSpc2.1软件,分别计算两种分子标记数据的系数矩阵,采用UPGMA法对所选材料进行聚类分析。【结果】SRAP共检测出了134个多态性位点,每组合的多态性条带数从2～8不等,平均每个引物组合产生2.91个多态性位点,表明SRAP能检测出较多的遗传位点,能够较好地反映海岛棉的遗传多样性。RGA标记共扩增出了46个多态性位点,每组合的多态性条带数从2～5不等,平均每个引物组合产生2.08个多态性位点,表明RGA作为一种分子标记也能检测出较多的遗传位点,能够较好地反映海岛棉在抗病方面的遗传多样性。【结论】SRAP聚类结果基本与品种系谱来源一致,RGA聚类基本与材料的抗病水平一致。分子标记在鉴别品种和品种遗传多样性研究方面具有重要作用。
【Objective】 The genetic diversity of Xinjiang self-bred Xinhai series was studied. 【Method】 Polymorphic sites were detected by SRAP and RGA markers. Coefficient matrices of two molecular marker data were calculated by NTSYSpc2.1 software. UPGMA was used to cluster the selected materials. 【Result】 A total of 134 polymorphic loci were detected in SRAP. The number of polymorphic bands in each group ranged from 2 to 8, with an average of 2.91 polymorphic loci per primer combination, indicating that SRAP could detect More genetic loci, can better reflect the genetic diversity of island cotton. RGA markers amplified a total of 46 polymorphic loci, each combination of polymorphic bands ranging from 2 to 5, the average combination of each primer generated 2.08 polymorphic sites, indicating that RGA as a Molecular markers can also detect more genetic loci, which can better reflect the genetic diversity of island cotton in disease resistance. 【Conclusion】 The results of SRAP clustering are basically consistent with those of pedigree pedigree. The RGA clustering is consistent with the disease resistance of the materials. Molecular markers play an important role in the identification of genetic diversity of cultivars and cultivars.