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本文进行了小麦和裸燕麦悬浮细胞原生质体的电融合,并基于双亲失活(用IOA处理受体小麦原生质体,用γ-射线照射供体裸燕麦细胞系),获得可能的杂种愈伤组织。对7块愈伤组织进行了乙醇脱氢酶(Adh)同工酶筛选,发现5块表现出双亲特征酶带。对3个杂种细胞系进行5种同工酶分析,证实它们均为稳定的不对称体细胞核杂种细胞系;它们表现出小麦的完整谱带和裸燕麦的部分谱带。对2个杂种细胞系及亲本的核糖体DNA Southern分析结果表明只有一个杂种细胞系(HB 95)含有双亲的全部谱带。细胞学观察表明,2个杂种细胞系的染色体数目均显著高于双亲。从杂种细胞系HB 94中分化出叶原基等分化结构。Adh同工酶分析表明,这些分化结构具有和母体细胞系完全相同的杂种谱带。
In this paper, electroplating of protoplasts from suspension cells of wheat and naked oats was performed and possible hybrid callus was obtained based on in-parent inactivation (recipient wheat protoplasts treated with IOA and donor naked oat cell lines irradiated with γ-rays) . Seven callus tissues were screened by isozyme of alcohol dehydrogenase (Adh) and found that five showed characteristic bands of parents. Five isozyme analyzes of the three hybrid cell lines confirmed that they were both stable asymmetric somatic cell hybrid cell lines; they showed a complete band of wheat and a partial band of naked oats. Southern analysis of the ribosomal DNA of two hybrid cell lines and their parents showed that only one hybrid cell line (HB95) contained the entire band of both parents. Cytological observations showed that the number of chromosomes in the two hybrid cell lines was significantly higher than that of the parents. Differentiation of leaf primordia and other differentiated structures from the hybrid cell line HB 94. Adh isozymes analysis showed that these differentiated structures have exactly the same hybrid bands as the maternal cell line.