目的:建立测定骨肽注射液中钙含量的方法,并对其进行方法学验证。方法:样品采用混合酸消化后导入原子吸收分光光度计,经火焰原子化,于422.7nm波长处测定。结果:钙的检测浓度在0～8μg.mL-1范围内与吸光度呈良好的线性关系(r=0.9997);平均加样回收率为100.34%,RSD=1.97%(n=9)。结论:本方法灵敏、准确、可靠,可用于检测骨肽注射液中钙的含量。 Objective: To establish a method for the determination of calcium content in bone peptide injection and verify the method. Methods: The sample was digested with mixed acid and then introduced into atomic absorption spectrophotometer. The sample was atomized by flame and measured at the wavelength of 422.7nm. Results: The concentration of calcium in the range of 0 ~ 8μg.mL-1 showed a good linear relationship with the absorbance (r = 0.9997). The average recoveries were 100.34% and RSD = 1.97% (n = 9). Conclusion: The method is sensitive, accurate and reliable and can be used to detect the content of calcium in bone peptide injection.