论文部分内容阅读
目的了解宁波口岸进口水产品中副溶血性弧菌的耐药性和毒力基因分布情况。方法将分离自宁波口岸进口水产品中的129株副溶血性弧菌作为研究对象,利用K-B纸片扩散法测定细菌对15种抗生素的耐药性,使用PCR方法检测副溶血性弧菌的4个毒力基因(tdh、tlh、trh、toxR)。结果菌株对氨苄西林、羧苄西林、头孢拉定的耐药率分别为90.70%、98.45%和51.17%;对卡那霉素、头孢氨苄、头孢唑啉、头孢呋辛的耐药率分别为1.55%、13.18%、2.33%、20.16%,中敏感率分别为33.33%、52.71%、46.51%、40.31%;其他8种抗生素(四环素、强力霉素、阿米卡星、庆大霉素、头孢哌酮、氧氟沙星、氯霉素、复方新诺明)则比较敏感;毒力基因trh,tdh,tlh和toxR的携带率分别为0%、0.78%、100%和100%。结论强力霉素、庆大霉素、复方新诺明是治疗由进口水产品引起的副溶血性弧菌感染的首选抗生素;进口水产品中副溶血性弧菌的主要致病毒力基因携带率比较低。
Objective To understand the drug resistance and virulence genes distribution of Vibrio parahaemolyticus in imported aquatic products in Ningbo Port. Methods 129 strains of Vibrio parahaemolyticus isolated from imported aquatic products in Ningbo Port were used as research objects. The bacterial resistance to 15 antibiotics was determined by KB disk diffusion method. The Vibrio parahaemolyticus 4 Virulence genes (tdh, tlh, trh, toxR). Results The resistance rates to ampicillin, carbenicillin and cefradine were 90.70%, 98.45% and 51.17%, respectively. The rates of resistance to kanamycin, cephalexin, cefazolin and cefuroxime were 1.55% , 13.18%, 2.33% and 20.16%, respectively. The sensitivity rates were 33.33%, 52.71%, 46.51% and 40.31% respectively. The other 8 antibiotics (tetracycline, doxycycline, amikacin, gentamycin, cefoperazone Ketones, ofloxacin, chloramphenicol, and cotrimoxazole) were more sensitive. The carrying rates of virulence genes trh, tdh, tlh and toxR were 0%, 0.78%, 100% and 100%, respectively. Conclusion Doxycycline, gentamicin and cotrimoxazole are the first choice antibiotics for the treatment of Vibrio parahaemolyticus caused by imported aquatic products. The virulence factors of Vibrio parahaemolyticus in imported aquatic products Lower.