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目的 观察胰岛素对脑缺血再灌注损伤的治疗作用 ,并探讨其作用机制。方法 用线栓法建立大鼠脑缺血再灌注模型 ,根据胰岛素的给药时间不同共分成 2大组 ,第 1大组再分为A、B、C、D 4组 ,第 2大组再分为 2组。在第 1大组检测各组不同时限的血糖 ,测量计算脑梗死灶体积 ,并进行神经功能缺损评分 ;在第 2大组采用TUNEL法原位标记DNA片段 ,检测TUNEL阳性细胞的变化。结果 在 6h内给予胰岛素治疗可使神经功能缺陷评分显著降低 ,脑梗死灶体积缩小 ,TUNEL阳性细胞也明显减少 (P <0 .0 5 )。结论 早期应用胰岛素能减轻脑缺血再灌注损伤 ,减轻再灌注后细胞损伤可能是其作用机制之一。
Objective To observe the therapeutic effect of insulin on cerebral ischemia-reperfusion injury and to explore its mechanism. Methods The rat model of cerebral ischemia-reperfusion was established by thread occlusion. According to the different administration time of insulin, the rats were divided into two groups. The first group was divided into A, B, C and D 4 groups. The second group Divided into two groups. In the first group, blood glucose was measured at different time points in each group, volume of cerebral infarction was calculated and neurological deficit score was calculated. In the second group, DNA fragments were labeled by TUNEL in situ to detect the changes of TUNEL positive cells. Results Insulin treatment within 6h significantly reduced neurological deficit score, reduced infarct size and decreased TUNEL positive cells (P <0.05). Conclusion Early application of insulin can reduce cerebral ischemia-reperfusion injury and reduce cell injury after reperfusion may be one of the mechanisms.