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目的：研究转换酶抑制剂卡托普利（Ｃａｐ）和依那普利拉（Ｅｎａ）对ＳＨＲ和ＷＫＹ大鼠心肌细胞内游离Ｃａ２＋浓度的影响．方法：用荧光探针Ｆｕｒａ２ＡＭ结合计算机图象处理技术测定分离心肌细胞内游离Ｃａ２＋浓度．结果：ＳＨＲ心肌细胞内游离Ｃａ２＋浓度（１７４±５ｎｍｏｌ·Ｌ－１）较ＷＫＹ大鼠（１４８±１５ｎｍｏｌ·Ｌ－１）高（Ｐ＜００１）．Ｃａｐ和Ｅｎａ能明显降低ＳＨＲ心肌细胞内游离Ｃａ２＋浓度（分别为１６１±１１和１６６±７ｎｍｏｌ·Ｌ－１，Ｐ分别＜００５），但对ＷＫＹ的无影响（Ｐ＞００５）．两药均能明显降低ＮＥ和ＡｎｇＩ引起的ＳＨＲ和ＷＫＹ大鼠心肌细胞内Ｃａ２＋升高，同时也能明显降低ＫＣｌ引起的ＳＨＲ细胞内Ｃａ２＋升高（Ｐ＜００５），但对ＷＫＹ大鼠的无明显影响（Ｐ＞００５）．结论：Ｃａｐ和Ｅｎａ对病理性电压依赖性Ｃａ２＋通道有直接抑制作用．
AIM: To investigate the effects of converting enzyme inhibitors Cap and ena on intracellular free Ca2 + concentration in SHR and WKY rats. Methods: Fluorescence probe Fura2 AM combined with computer image processing technology to measure the concentration of free Ca2 + in isolated myocardial cells. Results: The intracellular free Ca2 + concentration in SHR was (174 ± 5nmol·L-1) higher than that in WKY rats (148 ± 15nmol·L-1) (P <001). Cap and Ena significantly decreased the intracellular free Ca2 + concentration in SHR cells (161 ± 11 and 166 ± 7 nmol·L-1, P <005, respectively), but had no effect on WKY (P> 005). Both drugs can significantly reduce NE and AngI-induced SHR and WKY rat myocardial cell increased Ca2 +, but also can significantly reduce KCl-induced SHR intracellular Ca2 increased (P <0 05), but WKY rats No significant effect (P> 0 05). Conclusion: Cap and Ena have a direct inhibitory effect on pathological voltage-dependent Ca2 + channels.