鉴于ＴＰＯ 在体内长期表达会给机体带来一些负面影响，以及在较长期作用模式下，机体血小板的生成能力与ＴＰＯ 在体内水平的不一致，本文对免疫因子与ＴＰＯ 表达及其生物活性间的关系进行了研究。在ＴＰＯ ｃＤＮＡ 转移到体内后，血液中ＩＦＮ－γ、ＴＮＦ－ α和ＩＬ－２ 迅速升高。其中，ＩＦＮ－γ与血小板计数呈明显负相关， 在血小板计数回落后保持在对照水平的 ９～１２ 倍；ＴＮＦ－α仅在第 １ 周内与之成正相关，且可达对照水平的１２０ 倍，此后局限于对照水平；ＩＬ－２ 升高幅度较小，与ＴＰＯ 的变化相一致。另外，血液中Ⅱ型组织相容性抗原分子也呈升高趋势，与ＩＬ－２ 的变化类似；而Ⅰ型分子与ＴＮＦ－ α的变化相似。结合基因转移后的组织学特点，可以认为ＩＦＮ－γ在血小板生成过程中起主要的逆向调节作用，而机体出现了以巨噬细胞、内皮细胞和Ｔ 淋巴细胞活化为特点的免疫学改变 In view of the long-term expression of TPO in vivo will have some negative effects on the body, and in the longer-term mode of action, the body’s platelet production capacity and TPO in vivo levels of inconsistency, the immune factors and TPO expression and biological activity relationship Were studied. After transfection of TPO cDNA into the body, the levels of IFN-γ, TNF-α and IL-2 in blood are rapidly increased. Among them, IFN-γ was negatively correlated with platelet count, remained 9 to 12 times of the control level after the platelet count fell back; TNF-α was positively correlated with it only in the first week and reached 120 times of the control level , Then confined to the control level; IL-2 increased less, consistent with changes in TPO. In addition, blood type Ⅱ histocompatibility antigen molecules also showed an upward trend similar to the changes of IL-2; and type Ⅰ molecules and TNF-α changes are similar. Combined with the histological features of gene transfer, it is thought that IFN- [gamma] plays a major inverse regulatory role in platelet production and that the body presents immunological changes characterized by the activation of macrophages, endothelial cells, and T lymphocytes