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从糖专一性相同或不同的24种凝集素中筛选出SML、MDL、CKL和PHA-S四种能与人精子发生强烈反应的凝集素,浓度为0.98μg/ml时就能使精子凝集成块,6 h后,凝集团块也不分散,用机械方法分散团块所得精子已完全失去活动能力;其凝集作用可被凝集素各自的抑制糖所抑制;用FITC标记的SML、CKL、MDL与正常人精子作用后,在荧光显微镜下可观察到各凝集素受体的分布部位各不相同;用去垢剂增溶精子,Brij 58的增溶效果最好,增溶的膜蛋白可强烈地抑制凝集素对兔红细胞的凝集作用。
Four kinds of agglutinins, SML, MDL, CKL and PHA-S, which can react strongly with human spermatozoa were screened from 24 kinds of lectins with the same or different saccharide specificity. When the concentration is 0.98μg / ml, sperm can be aggregated Aggregation, 6 h after the agglomeration is not dispersed, mechanically scattered clumps obtained sperm has been completely lost activity; its agglutination can be inhibited by the inhibition of each of the lectin; FITC-labeled SML, CKL, MDL and normal human sperm role, the distribution of each lectin receptor can be observed under a fluorescence microscope different parts; with detergent sperm solubilization, Brij 58 the best solubilization, solubilized membrane protein can Strongly inhibit lectin agglutination of rabbit erythrocytes.