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目的为了适应教学时大批量使用的组织切片要求，探索提高淋巴结组织石蜡切片整体质量的方法。方法取门诊及住院患者活检淋巴结组织，对制片过程中组织取材、固定、脱脂、脱水与透明、浸蜡、包埋、切片、染色及封固等步骤进行改进。结果淋巴结组织结构完整，染色清晰。结论淋巴结组织石蜡切片制作需注意各步骤的控制，通过改进技术有效地提高了教学切片的质量。“,”Objective: To investigate the quality improving frozen section techniques and methods. Methods and materials: Fresh lymph node tissue sampling obtained from the clinic patients, and methods in the preparing of formalin fixed and paraf in-embedded lymph node tissue sections have been ameliorated include tissue fixation, defat ing etc. Results: The lymph node section were complete, uniform thickness and died bright. Conclusion: High quality of tissue sections could be obtained through the methods ameliorated and the ef iciency was improved.