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目的:建立紫外检测高效液相色谱方法测定动物样本中纳曲酮浓度。方法:采用YWG-C18色谱柱（4.6mm×150mm,5μm）,UV1000紫外检测器,甲醇-乙腈-磷酸盐缓冲液（pH4.0）（15:10:75）为流动相,流速:1.0mL·min-1;以乙酰苯胺为内标,样本经有机相提取纯化后进样,检侧波长230nm。结果:测定血浆中药物浓度时的线性范围 5～40ng·mL-1,回收率91.4％～94.5％,日内误差（RSD）<12％,日间误差（RSD）<7.5％;肝组织测定时线性范围5～200ng·mL-1,回收率78.8％～82.3％,日内误差（RSD）<17％,日间误差（RSD）<4.5％;肾组织测定时线性范围5～200ng·mL-1,回收率79.4％～83.4％,日内误差（RSD）<8.1％,日间误差（HSD）<8.2％。结论:本方法快速、准确、重复性好,可以满足临床和动物试验样品测定的要求。
Objective: To establish a UV detection high performance liquid chromatographic method for the determination of naltrexone in animal samples. Methods: The mobile phase was YWG-C18 column (4.6mm × 150mm, 5μm), UV1000 UV detector and methanol-acetonitrile-phosphate buffer (pH4.0) Min-1; acetanilide as internal standard, the sample was extracted by the organic phase extraction, the detection wavelength 230nm. Results: The linear range was 5 ~ 40ng · mL-1, the recovery rate was 91.4% -94.5%, the intraday error (RSD) <12% and the daytime error (RSD) <7.5% The linear range was 5 ~ 200ng · mL-1, the recovery was 78.8% -82.3%, the intra-day error <17%, the daytime error <4.5% , The recoveries ranged from 79.4% to 83.4%, the daily error (RSD) <8.1% and the daytime error (HSD) <8.2%. Conclusion: The method is rapid, accurate and reproducible, which can meet the requirements of clinical and animal test samples.