目的　在单个细胞水平 ,观测亚溶破补体膜攻击复合物 (MAC)诱导的血管内皮细胞 [Ca2 + ]i的动态变化。方法将原代培养的人脐静脉内皮细胞 ,以Fluo 4 /AM(1～ 6 μmol/L)和 0 .0 2 %PluronicF 12 7进行细胞钙荧光指示剂负载后 ,于 0℃组装亚溶破剂量的MAC。在 37℃条件下 ,以LSCM实时监测MAC沉积引起的胞浆钙荧光强度的变化。结果　MAC沉积后 ,多数细胞的钙荧光强度迅速增强 ,以后随时间的推移 ,升高的钙荧光逐渐下降 ,直至恢复至静息水平。定量分析选定的胞浆区域钙荧光强度的变化发现 ,不同细胞在 [Ca2 + ]i 的变化高度、持续时间和胞内钙振荡的特征等方面存在异质性。结论　MAC沉积到内皮细胞表面后 ,可诱导胞浆游离钙离子的浓度增高 ,且不同细胞 [Ca2 + ]i 的变化存在明显的异质性 Objective To observe the dynamic changes of [Ca2 +] i in vascular endothelial cells induced by macrophage infiltration membrane complex (MAC) at the single cell level. Methods Primary cultured human umbilical vein endothelial cells were loaded with Fluo 4 / AM (1 ~ 6 μmol / L) and 0.2% Pluronic F 12 7, and then assembled at 0 ℃. The dose of MAC. The changes of fluorescence intensity of cytoplasmic Ca 2+ induced by MAC deposition were monitored by LSCM in real time at 37 ℃. Results After MAC deposition, the calcium fluorescence intensity of most cells rapidly increased. Afterwards, the increased calcium fluorescence gradually decreased until the resting level was restored. Quantitative analysis of changes in the fluorescence intensity of selected cytoplasmic regions found heterogeneity in the variation of [Ca2 +] i, duration, and intracellular calcium oscillations among different cells. Conclusion After MAC was deposited on the surface of endothelial cells, the concentration of free calcium ion in cytoplasm could be induced to increase, and there was obvious heterogeneity in the changes of [Ca2 +] i in different cells