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目的 探讨内源性一氧化碳 (CO)在大鼠肢体缺血再灌注 (I/ R)致远隔多器官氧化性损伤中的作用机制。方法 将 6 4只大鼠随机分为 4组 :假手术 (Sham )组 ;Sham+特异性血红素氧化酶阻断剂—锌原卟啉 (Zn PP)组 ;肢体缺血 2小时和再灌注 4小时 (I/ R)组 ;I/ R+Zn PP组。测定各组心、肺、肝和肾组织匀浆中丙二醛 (MDA )含量、超氧化物歧化酶 (SOD)活性及血液内碳氧血红蛋白 (COHb)的变化 ,观察动物 2 4小时存活率。结果 与 Sham组相比 ,I/ R组各脏器 MDA含量及血液内 COHb水平均显著增高 ,组织中 SOD活性和动物 2 4小时存活率显著降低 ,有统计学意义(P<0 .0 5 ) ;I/ R+Zn PP组与 I/ R组相比各脏器 MDA含量进一步增高 ,血液内 COHb水平、组织中 SOD活性和动物的2 4小时存活率显著降低 ,也有统计学意义 (P<0 .0 5 )。结论 肢体缺血再灌注可导致多器官的氧化性损伤 ,并使 CO产生增多 ,后者在大鼠抗缺血再灌注所致的远隔多器官损伤中具有重要作用。
Objective To investigate the mechanism of endogenous carbon monoxide (CO) in the oxidative damage of multiple organs caused by limb ischemia-reperfusion (I / R) in rats. Methods Sixty-four rats were randomly divided into 4 groups: Sham group; Sham + specific heme oxygenase inhibitor-Zn PP group; 2 h limb ischemia and reperfusion 4 Hour (I / R) group; I / R + Zn PP group. The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and carboxyhemoglobin (COHb) in heart, lung, liver and kidney homogenate were measured. The survival rate of 24 hours . Results Compared with Sham group, the content of MDA and the level of COHb in blood of I / R group were significantly increased, and the activity of SOD and the survival rate of 24 hours in animals were significantly decreased (P <0.05) ); Compared with I / R group, the content of MDA in I / R + Zn PP group was significantly higher than that in I / R group. The level of COHb in blood, the activity of SOD in tissues and the 24-hour survival rate of animals were significantly decreased <0 .0 5). Conclusion Limb ischemia-reperfusion can lead to oxidative damage in multiple organs and increased CO production, which plays an important role in the protection of rats from multiple organ damage caused by ischemia-reperfusion.