论文部分内容阅读
目的:观察5-脱氧杂氮胞苷(5-aza-CdR)对HepG2细胞生长抑制及beclin1表达的影响,以探讨其抗肿瘤发生的潜在机制。方法:采用四甲基偶氮唑盐(MTT)法检测5-aza-CdR对HepG2细胞的生长抑制;用相差显微镜观察不同药物浓度下不同时间段的肝癌细胞形态学改变;采用RT-PCR法和Western blot法检测5-Aza-CdR对抑癌基因beclin1的mRNA和蛋白表达的影响。结果:5-aza-CdR可抑制HepG2细胞生长,呈剂量依赖性,并上调beclin1的mRNA和蛋白的表达。结果:显示102.4umol/L5-aza-C-dR作用72小时细胞增殖抑制率最高,可达(84.3±3.31)%,beclin1的mRNA和蛋白表达上调最明显,与对照组相比差异有统计学意义。结论:5-aza-CdR可抑制HepG2细胞增殖,其机制可能是通过恢复某些抑癌基因的表达,上调beclin1的mRNA和蛋白表达。
OBJECTIVE: To observe the effect of 5-aza-CdR on the growth inhibition and the expression of beclin1 in HepG2 cells and to explore the potential mechanism of its anti-tumor effect. Methods: The inhibitory effect of 5-aza-CdR on HepG2 cells was detected by MTT assay. The morphological changes of hepatoma cells were observed by phase-contrast microscopy at different time points. And Western blot were used to detect the effect of 5-Aza-CdR on the mRNA and protein expression of beclin1. Results: 5-aza-CdR inhibited the growth of HepG2 cells in a dose-dependent manner and up-regulated the mRNA and protein expression of beclin1. The results showed that the inhibition rate of the cell proliferation was the highest (84.3 ± 3.31)% after 72.4 hours of 102.4umol / L 5-aza-C-dR treatment, and the mRNA and protein expression of beclin1 were the most obvious up-regulation compared with the control group significance. CONCLUSION: 5-aza-CdR can inhibit the proliferation of HepG2 cells by up-regulating the mRNA and protein expression of beclin1 by restoring the expression of some tumor suppressor genes.