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脐血作为造血于/祖细胞的来源具有诸多优点,应用前景广泛.采用多种造血生长因子组合,建立体外大量扩增脐血于/祖细胞的培养体系,已逐渐成为人们关注的热点.本研究动态观察了脐血单个核细胞(MNC)经不同造血生长因子组合刺激的增殖效应.结果显示,①脐血MNC培养至11～20d时,达到增殖高峰,21d后细胞增殖明显变缓.20d时,不同的造血生长因子组合对脐血造血于/祖细胞扩增倍数不同,结果如下,(a)rhIL-1+rhIL-3+rhIL-6+rhG-CSF+rhGM-CSF+rhSCF组合使有核细胞总数增殖为初始的43倍;
Umbilical cord blood as a source of hematopoietic progenitor cells has many advantages and broad application prospects.Using a variety of hematopoietic growth factor combinations to establish a culture system that extensively amplifies umbilical cord blood progenitor cells in vitro has become a hot topic of concern. The study dynamically observed the proliferative effect of cord blood mononuclear cells (MNC) stimulated by different hematopoietic growth factor combinations.The results showed that: (1) When umbilical cord blood MNC cultured to 11 ~ 20d, the proliferative peak reached its peak, and the cell proliferation slowed down significantly after 21d (A) rhIL-1 + rhIL-3 + rhIL-6 + rhG-CSF + rhGM-CSF + rhSCF combined with different combinations of hematopoietic growth factors on cord blood hematopoietic progenitor proliferation ratio The total number of nucleated cells proliferation was 43 times the initial;