论文部分内容阅读
目的研究中药抗脂方调节糖脂代谢,改善非酒精性脂肪性肝炎的作用。方法将SD大鼠随机分为正常对照组(A组)、脂肪性肝炎模型组(B组)、脂肪性肝炎给予易善复组(C组)和脂肪性肝炎给予抗脂方组(D组)。除正常对照组外,其余各组以高脂饲料喂养,同时皮下注射CCl4造模。造模同时灌胃给药8周。以常规方法检测肝功能、生化指标、肝组织病理染色和HOMA-IR;采用ELISA法检测脂联素(ADP/Acrp3)、过氧化物酶体增殖物激活受体α(PPAR-α)、肿瘤坏死因子-α(TNF-α)、空腹胰岛素(FINS)、游离脂肪酸(FFA)和蛋白酪氨酸激酶(PTK);用Western blotting法检测胰岛素信号通路相关蛋白表达。结果与脂肪性肝炎模型组比较,脂肪性肝炎给予抗脂方组明显改善了非酒精性脂肪性肝炎大鼠的肝功能,逆转了其相关的病理特征及生化指数,差异有统计学意义(P<0.05,P<0.01);同时,脂肪性肝炎给予抗脂方组大鼠FPG、FINS、HOMA-IR降低(P<0.01),PTK、PPAR-α、ADP升高(P<0.01),TNF-α降低(P<0.01),胰岛素受体IR增加(P<0.01),IRS1、IRS2升高(P<0.05),PI3K表达及Akt的磷酸化表达明显升高(P<0.05),p38的磷酸化水平明显降低(P<0.01),从而增加了大鼠肝细胞对胰岛素的敏感性,改善了胰岛素信号转导。结论中药抗脂方具有明显改善非酒精性脂肪性肝炎的作用,其相关的机制可能与抗脂方调节肝脏的糖脂代谢、改善胰岛素信号通路有关。
Objective To study the effect of traditional Chinese medicine Anti-obesity prescription in regulating glucose and lipid metabolism and improving non-alcoholic steatohepatitis. Methods SD rats were randomly divided into normal control group (group A), steatohepatitis model group (group B), steatohepatitis group (group C) and steatohepatitis group (group D) ). Except the normal control group, the other groups were fed with high-fat diet and injected with CCl4 subcutaneously. Modeling at the same time gavage for 8 weeks. The liver function, biochemical indexes, histopathological staining of liver tissue and HOMA-IR were detected by routine methods. The expressions of adiponectin (ADP / Acrp3), peroxisome proliferator activated receptor α (PPAR-α) TNF-α, FINS, FFA and PTK. Western blotting was used to detect the expression of insulin signaling pathway related proteins. Results Compared with steatohepatitis model group, steatohepatitis group was significantly improved liver function in non-alcoholic steatohepatitis rats, reversing its pathological features and biochemical index, the difference was statistically significant (P (P <0.05, P <0.01). At the same time, FPG, FINS and HOMA-IR in the group of anti-ZhiFang were decreased (P <0.01), PTK and PPAR- (P <0.01), insulin receptor IR increased (P <0.01), IRS1, IRS2 increased (P <0.05), PI3K expression and phosphorylation of Akt increased significantly (P <0.05) Phosphorylation was significantly decreased (P <0.01), thereby increasing the sensitivity of rat hepatocytes to insulin and improving insulin signaling. Conclusion Anti-obesity prescription can significantly improve the non-alcoholic steatohepatitis, and its related mechanism may be related to the anti-obesity prescription regulating hepatic glycolipid metabolism and improving the insulin signaling pathway.