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目的探讨人工风寒湿与风湿热环境影响大鼠胶原诱导性关节炎（ＣＩＡ）发病的机理。方法在ＣＩＡ的诱导阶段，给予风寒湿或风湿热刺激，连续观察大鼠关节炎症积分。免疫后第３０天取血，ＥＬＩＳＡ法检测血清胶原抗体水平，放免法检测血清雌二醇（Ｅ２）及三碘甲状腺原氨酸（Ｔ３）含量。结果１．风寒湿、风湿热均可显著提高雌性鼠的胶原抗体水平，但风寒湿显著降低雌性鼠关节炎症积分。２．寒湿显著升高雌性鼠血清Ｅ２及Ｔ３含量。３．雌性鼠风寒湿ＣＩＡ、风湿热ＣＩＡ及雄性鼠单纯ＣＩＡ组关节炎症积分与Ｅ２含量呈显著负相关。结论促进雌性鼠Ｅ２的分泌可能是风寒湿抑制雌性鼠ＣＩＡ炎症程度的主要原因
Objective To investigate the mechanism of collagen-induced arthritis (CIA) induced by artificial wind, cold and dampness and rheumatic fever in rats. Methods In the inducing phase of CIA, cold or humid or rheumatic fever was given to the rats to observe the joint inflammation scores continuously. Blood samples were collected on the 30th day after immunization. Serum collagen antibody levels were measured by ELISA. Serum estradiol (E2) and triiodothyronine (T3) contents were determined by radioimmunoassay. Results 1. Cold and dampness, rheumatic fever can significantly increase the level of collagen antibody in female mice, but cold and wet significantly reduce the female rats joint inflammation score. 2. Cold and dampness significantly increased female rats serum E2 and T3 content. 3. Female rats with cold wet CIA, rheumatic fever CIA and male rats with simple CIA group joint inflammation score and E2 content was significantly negatively correlated. Conclusions The promotion of E2 secretion in female rats may be the main reason for the degree of inflammation of CIA in female mice inhibited by wind-cold wet