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以前期获得的脱除番茄不孕病毒(TAV)的‘怀白菊’试管苗为试材,从同工酶谱和DNA分子简单序列间重复(ISSR)多态性方面分析其遗传稳定性,在此基础上,对其继代培养过程中叶片中的活性氧代谢进行了研究。结果表明,TAV脱除没有改变‘怀白菊’叶片中SOD、POD同工酶谱带及ISSR扩增条带的数目,但相应同工酶谱带亮度增强;也没有改变‘怀白菊’试管苗继代培养过程中膜脂过氧化相关指标(O2ˉ·生成速率及H2O2和MDA含量)和保护系统相关指标(保护酶SOD、POD、CAT、APX和GR,抗氧化物质GSH和As A)的变化趋势,但却显著降低了相同时间点膜脂过氧化相关指标的绝对值,降幅最大值超过30%(O2ˉ·生成速率、MDA含量),显著增加了保护酶活性及抗氧化物质的含量,增幅最大值超过了50%(APX活力、GSH含量)。以上说明,TAV脱除不仅没有改变‘怀白菊’的遗传稳定性,而且降低了其活性氧代谢水平,提高了其抗氧化能力。
Based on the genetic stability of isozymes and simple inter-sequence repeat (ISSR) polymorphism of DNA molecules obtained from pre-hatched tomato embryo virus (TAV) On this basis, the metabolism of reactive oxygen species in leaves during its subculture was studied. The results showed that the removal of TAV did not change the number of SOD, POD isozymes and ISSR bands in ’Huai Bai Ju’ leaves, but the brightness of the corresponding isozyme bands was enhanced. Changes of membrane lipid peroxidation (O2ˉ · generation rate, H2O2 and MDA content) and changes of protective system (SOD, POD, CAT, APX and GR, antioxidants GSH and As A) during subculture But significantly reduced the absolute value of the relative indices of membrane lipid peroxidation at the same time point, the maximum value of which exceeded 30% (O2ˉ · generation rate and MDA content), significantly increased the activity of protective enzymes and the content of antioxidants The maximum value exceeded 50% (APX activity, GSH content). The above description, TAV removal not only failed to change the genetic stability of ’Huai Baiju’, but also reduce its level of active oxygen metabolism and improve its antioxidant capacity.