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用ＰＣＲ／ＲＦＬＰ技术对北京郊区１１个旅游景点的媒介蜱及小哺乳动物进行斑点热群立克次体的检测，并进行了病原体分离和鉴定。结果从北京航空博物馆附近地区收集的刺猬和其体表寄生的中华革蜱中分离出２株斑点热群立克次体，分别命名为ＢＪＨ－９５株和ＢＪ－９５株，经ＳＤＳ－ＰＡＧＥ、Ｗｅｓｔｅｒｎｂｌｏｔ和ＰＣＲ／ＲＦＬＰ技术进行了鉴定认为是西伯利亚立克次体。结果证明了该立克次体在蜱和动物宿主间呈水平传播，并且表明在被调查的旅游景点中除航空博物馆附近地区外，大多数景点基本上是北亚热的安全区。在刺猬中分离出西伯利亚立克次体为国内、外首次报道
The ticks and small mammals in 11 tourist spots in Beijing suburbs were detected by PCR / RFLP, and the pathogens were isolated and identified. Results Two spotted fever group Rickettsia were isolated from the hedgehog collected from the area around Beijing Aeronautical Museum and their body surface parasitism, and were named BJH-95 and BJ-95, respectively. SDS-PAGE, Western blot and PCR / RFLP were identified as Siberian Rickettsia. The results demonstrated that the rickettsia was transmitted horizontally between ticks and animal hosts and showed that most of the attractions in the surveyed tourist spots, except the area near the Aviation Museum, were essentially North Asia safe areas. Siberian rickettsia was isolated from hedgehog for the first time, both at home and abroad