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本研究以 17个杂交水稻亲本、3个新株型株系和 2 4个光壳稻、爪哇稻品种为DNA样品来源 ,通过RFLP(限制性片段长度多态性 ,RestrictionFragmentLengthPolymorphism ,简称RFLP)标记技术 ,研究光壳稻和爪哇稻及其与温带粳稻之间的关系 ,探索RFLP标记水稻亲本遗传差异在杂交稻育种中利用的可能性。研究结果表明 :42个探针共产生 6 9个不同的限制性片段 ,其中 2 0个 (占 47.6 % )探针显示 47个 (占 6 8.1% )多态性片段 ,至少在 2个基因型间存在差异。每个具有多态性片段分别以 1和0记录存在与否。由RFLP数据计算的Nei’s遗传距离 ,创建聚类树状图。聚类分析结果表明 ,籼稻和粳稻容易被分开 ,温带粳稻又容易与光壳稻、爪哇稻分开 ,但光壳稻和爪哇稻混合聚在一起 ,光壳稻与温带粳稻之间的遗传距离要比爪哇稻与温带粳稻之间的遗传距离大。根据聚类图发现温带粳稻亚群内杂种优势较弱 ,亚群间即生态群间的杂种优势较强 ,群间即籼、粳亚种间杂种优势更强。利用光壳稻、爪哇稻选育不同生态群方向的恢复系和不育系 ,已配组育成了强优势的杂交稻组合
In this study, 17 hybrid rice parents, 3 new plant-type lines and 24 light-shell rice varieties were used as DNA samples. RFLP (restriction fragment length polymorphism, RFLP) Studied the relationship between photoperiod-rice and Javanese-rice and its relationship with temperate japonica rice, and explored the possibility of utilizing the genetic differences of RFLP-labeled rice parents in hybrid rice breeding. The results showed that 42 probes produced a total of 69 different restriction fragments, of which 20 (47.6%) probes showed 47 polymorphic fragments (61.8%), at least 2 genotypes There are differences between. Each fragment with a polymorphism is recorded as 1 and 0 respectively. Nei’s genetic distance, calculated from RFLP data, was used to create a cluster tree. Cluster analysis showed that indica and japonica rice could be easily separated, and temperate japonica rice could be easily separated from husk rice and Javanese rice, however, the light-husk rice and Javanese rice mixed together, and the genetic distance between husk rice and temperate japonica rice was Compared with Java and temperate Japonica rice genetic distance between. According to the cluster diagram, it was found that the heterosis was weaker in the temperate japonica rice subgroups, and the heterosis was stronger in the subpopulation, that is, the heterosis between the indica and japonica subspecies. The use of light shell rice, Java paddy breeding of different ecotypes of the direction of the restorer lines and sterile lines, has been bred with a strong hybrid rice combinations